Fast events in protein folding
نویسندگان
چکیده
منابع مشابه
Fast events in protein folding initiated by nanosecond laser photolysis.
Initiation of protein folding by light can dramatically improve the time resolution of kinetic studies. Here we present an example of an optically triggered folding reaction by using nanosecond photodissociation of the heme-carbon monoxide complex of reduced cytochrome c. The optical trigger is based on the observation that under destabilizing conditions cytochrome c can be unfolded by preferen...
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Proteins fold in a time range of microseconds to minutes despite the large amount of possible conformers. Molecular dynamics simulations of a three-stranded antiparallel beta-sheet peptide (for a total of 12.6 microsec and 72 folding events) show that at the melting temperature the unfolded state ensemble contains many more conformers than those sampled during a folding event.
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Simplified Gō models, where only native contacts interact favorably, have proven useful to characterize some aspects of the folding of small proteins. The success of these models is limited by the fact that all residues interact in the same way, so that the folding features of a protein are determined only by the geometry of its native conformation. We present an extended version of a C α base...
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2.1. Turbulent Mixing 1838 2.2. Stopped-Flow Methods 1838 2.3. Continuous-Flow Techniques 1839 3. Detection Methods 1841 3.1. Tryptophan Fluorescence 1841 3.2. ANS Fluorescence 1842 3.3. Fluorescence Energy Transfer 1842 3.4. Continuous-Flow Absorbance 1843 3.5. Probes of Secondary Structure Formation 1844 3.6. Small-Angle X-ray Scattering 1844 3.7. Other Detection Methods 1844 4. Quenched-Flow...
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We describe a conceptual framework for understanding the way large RNA molecules fold based on the notion that their free-energy landscape is rugged. A key prediction of our theory is that RNA folding can be described by the kinetic partitioning mechanism (KPM). According to KPM a small fraction of molecules folds rapidly to the native state whereas the remaining fraction is kinetically trapped...
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ژورنال
عنوان ژورنال: Structure
سال: 1996
ISSN: 0969-2126
DOI: 10.1016/s0969-2126(96)00121-9